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Anti -160 kDa Neurofilament Medium Mouse mAb

價格: 在線咨詢
貨號:
KT011746
產品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
HMR/MR
購買數量:
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Product Information


Description

160 kDa Neurofilament Medium mouse monoclonal antibody

Protein full name

Neurofilament medium polypeptide

Synonyms

160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, Nef3, Nefm, NF-M, Nfm, 150kDa medium

Immunogen

Recombinant protein corresponding to Mouse NF-M

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Cytoskeleton

Predicted MW.

96 kDa

Observed MW.

150 kDa

Uniprot ID

P07197, P08553, P12839




Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 500-1: 1000

brain, cerebral cortex, hippocampus, spinal cord

IHC/IF

Mouse, Rat

1: 500-1: 4000

brain, spinal cord, epencephalon




Background


NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.




Images


圖片 1.png

Western blot analysis of NF-M (GB12763)  at dilution of 1: 1000


圖片 2.png

Immunohistochemistry analysis of paraffin-embedded mouse brain using NF-M (GB12763) at dilution of 1: 4000


圖片 3.png

Immunohistochemistry analysis of paraffin-embedded rat brain using NF-M (GB12763) at dilution of 1: 4000

圖片 4.png

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using NF-M (GB12763) at dilution of 1: 4000

圖片 5.png

Immunohistochemistry analysis of paraffin-embedded rat spinal cord using NF-M (GB12763) at dilution of 1: 4000

圖片 6.png Immunohistochemistry analysis of paraffin-embedded mouse epencephalon using NF-M (GB12763) at dilution of 1: 4000
圖片 7.png

Immunohistochemistry analysis of paraffin-embedded rat epencephalon using NF-M (GB12763) at dilution of 1: 4000

圖片 8.png

Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using NF-M (GB12763) at dilution of 1: 1000

圖片 9.png

Immunofluorescent analysis of paraformaldehyde-fixed rat brain using NF-M (GB12763) at dilution of 1: 1000


圖片 10.png

Immunofluorescent analysis of paraformaldehyde-fixed mouse spinal cord using NF-M (GB12763) at dilution of 1: 1000

圖片 11.png

Immunofluorescent analysis of paraformaldehyde-fixed rat spinal cord using NF-M (GB12763) at dilution of 1: 1000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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