recombinant SARS-CoV-2 nucleocapsid protein expressed inE.coil.
Isotype
IgG
Purity
Affinity purification
Predicted MW.
50 kDa
Observed MW.
50 kDa
Applications
Applications
Species
Dilution
Positive Tissue
WB
-----------
1:500-1:1000
Use at an assay dependent sample
Background
Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.
Images
Western blot analysis of SARS-CoV-2 nucleocapsid protein (GB122133) at dilution of 1: 1000.
Lane 1-4: recombination SARS-COV-2 nucleocapsid protein
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .